MutationTaster - study a chromosomal position

NEVER press reload or F5 - unless you want to start from the very beginning.
input seems to be ok - now mapping the variant to the different transcripts...
found 3 transcript(s)...
Querying Taster for transcript #1: ENST00000439785
Querying Taster for transcript #2: ENST00000303586
Querying Taster for transcript #3: ENST00000426813
MT speed 0 s - this script 2.676815 s

Results

genesymbol	prediction	probability	model	prediction problem	splicing	ClinVar	amino acid changes	variant type	dbSNP ID	protein length	file
ZNF30	polymorphism_automatic	1.23519619399381e-06	simple_aae		affected		R380K	single base exchange	rs1345658		show file
ZNF30	polymorphism_automatic	1.23519619399381e-06	simple_aae		affected		R379K	single base exchange	rs1345658		show file
ZNF30	polymorphism_automatic	1.23519619399381e-06	simple_aae		affected		R298K	single base exchange	rs1345658		show file

Taster files

mutation t@sting

documentation

Prediction

polymorphism

Model: simple_aae, prob: 0.999998764803806 (classification due to TGP/ExAC, real probability is shown anyway) (explain)

Summary

amino acid sequence changed
homozygous in TGP or ExAC
protein features (might be) affected
splice site changes

hyperlink

analysed issue

analysis result

name of alteration

no title

alteration (phys. location)

chr19:35435006G>AN/A show variant in all transcripts IGV

HGNC symbol

ZNF30

Ensembl transcript ID

ENST00000439785

Genbank transcript ID

NM_001099438

UniProt peptide

P17039

alteration type

single base exchange

alteration region

CDS

DNA changes

c.1139G>A
cDNA.1583G>A
g.17200G>A

AA changes

R380K Score: 26 explain score(s)

position(s) of altered AA
if AA alteration in CDS

380

frameshift

known variant

Reference ID: rs1345658

database	homozygous (A/A)	heterozygous	allele carriers
1000G	1177	913	2090
ExAC	18346	-3925	14421

regulatory features

Gene Associated, Regulatory Feature, Gene associated regulatory feature
H3K36me3, Histone, Histone 3 Lysine 36 Tri-Methylation
H3K9me3, Histone, Histone 3 Lysine 9 Tri-Methylation
H4K20me3, Histone, Histone 4 Lysine 20 Tri-Methylation
ZNF274, Transcription Factor, ZNF274 Transcription Factor Binding

phyloP / phastCons

	PhyloP	PhastCons
(flanking)	3.262	0.897
	1.006	0.723
(flanking)	-0.446	0.062

explain score(s) and/or inspect your position(s) in in UCSC Genome Browser

splice sites

effect	gDNA position	score	wt detection sequence	exon-intron border
Donor increased	17203	wt: 0.34 / mu: 0.50	wt: GAGAACCTTCAGTCG mu: GAAAACCTTCAGTCG	GAAC\|cttc
Donor marginally increased	17197	wt: 0.5566 / mu: 0.5774 (marginal change - not scored)	wt: ATGCGGGAGAACCTT mu: ATGCGGGAAAACCTT	GCGG\|gaga
Donor gained	17195	0.92	mu: GAATGCGGGAAAACC	ATGC\|ggga
Donor gained	17196	0.90	mu: AATGCGGGAAAACCT	TGCG\|ggaa

distance from splice site

880

Kozak consensus sequence altered?

N/A

conservation
protein level for non-synonymous changes

species

match

gene

alignment

Human

380

mutated

all conserved

380

Ptroglodytes

all conserved

ENSPTRG00000010822

379

Mmulatta

no homologue

Fcatus

no homologue

Mmusculus

no homologue

Ggallus

no homologue

Trubripes

no homologue

Drerio

no homologue

Dmelanogaster

no homologue

Celegans

no homologue

Xtropicalis

no homologue

protein features

start (aa)	end (aa)	feature	details
372	394	ZN_FING	C2H2-type 10.	lost
400	422	ZN_FING	C2H2-type 11.	might get lost (downstream of altered splice site)
428	450	ZN_FING	C2H2-type 12.	might get lost (downstream of altered splice site)
456	478	ZN_FING	C2H2-type 13.	might get lost (downstream of altered splice site)
484	506	ZN_FING	C2H2-type 14.	might get lost (downstream of altered splice site)
492	492	CONFLICT	T -> A (in Ref. 4; AAI42997).	might get lost (downstream of altered splice site)
493	493	CONFLICT	F -> L (in Ref. 2; CAE45802).	might get lost (downstream of altered splice site)
512	534	ZN_FING	C2H2-type 15.	might get lost (downstream of altered splice site)
540	562	ZN_FING	C2H2-type 16.	might get lost (downstream of altered splice site)
568	590	ZN_FING	C2H2-type 17.	might get lost (downstream of altered splice site)
596	618	ZN_FING	C2H2-type 18.	might get lost (downstream of altered splice site)

length of protein

normal

AA sequence altered

yes

position of stopcodon in wt / mu CDS

1875 / 1875

position (AA) of stopcodon in wt / mu AA sequence

625 / 625

position of stopcodon in wt / mu cDNA

2319 / 2319

poly(A) signal

N/A

conservation
nucleotide level for all changes - no scoring up to now

N/A

position of start ATG in wt / mu cDNA

445 / 445

chromosome

strand

last intron/exon boundary

704

theoretical NMD boundary in CDS

209

length of CDS

1875

coding sequence (CDS) position

1139

cDNA position
(for ins/del: last normal base / first normal base)

1583

gDNA position
(for ins/del: last normal base / first normal base)

17200

chromosomal position
(for ins/del: last normal base / first normal base)

35435006

original gDNA sequence snippet

CGGATGCAAGGAATGCGGGAGAACCTTCAGTCGTGCCTCAT

altered gDNA sequence snippet

CGGATGCAAGGAATGCGGGAAAACCTTCAGTCGTGCCTCAT

original cDNA sequence snippet

CGGATGCAAGGAATGCGGGAGAACCTTCAGTCGTGCCTCAT

altered cDNA sequence snippet

CGGATGCAAGGAATGCGGGAAAACCTTCAGTCGTGCCTCAT

wildtype AA sequence

MAHKYVGLQY HGSVTFEDVA IAFSQQEWES LDSSQRGLYR DVMLENYRNL VSMAGHSRSK
PHVIALLEQW KEPEVTVRKD GRRWCTDLQL EDDTIGCKEM PTSENCPSFA LHQKISRQKP
RECQEYGKTL CQDSKPVQHE RIHSSEKPNR CKECGKNFSN GHQLTIHQRL HVGEKPYKYE
KCGKAFISGS AFVKHGRIHT GEKPLKCKQC GKTISGSYQL TVHKSIHTGK KPYECGECGK
AFLVYGKLTR HQSTHTGEKP FGCEECGKAF STFSYLVQHQ RIHTSEKPYE CKECGKAFST
SSPLAKHQRI HTGEKPYECK ECGKSFTVYG QLTRHQSIHT GEKPFECKEC GKAFRLSSFL
HAHQRIHAEI KPYGCKECGR TFSRASYLVQ HGRLHTGEKP YECKECGKAF STGSYLVQHQ
RIHTGEKPYE CKECGKAFIS RHQLTVHQRV HTGEKPYECK ECGKAFRVHV HLTQHRKIHT
DVKPYECKEC GKTFSRASYL VQHSRIHTGK KPYECKECGK AFSSGSYLVQ HQRIHTGEKP
YECNKCGKAF TVYGQLIGHQ SVHTGEKPFE CKECGKAFRL NSFLTEHQRV HTGEKPFKCK
KCGKTFRYSS ALKVHLRKHM SVIP*

mutated AA sequence

MAHKYVGLQY HGSVTFEDVA IAFSQQEWES LDSSQRGLYR DVMLENYRNL VSMAGHSRSK
PHVIALLEQW KEPEVTVRKD GRRWCTDLQL EDDTIGCKEM PTSENCPSFA LHQKISRQKP
RECQEYGKTL CQDSKPVQHE RIHSSEKPNR CKECGKNFSN GHQLTIHQRL HVGEKPYKYE
KCGKAFISGS AFVKHGRIHT GEKPLKCKQC GKTISGSYQL TVHKSIHTGK KPYECGECGK
AFLVYGKLTR HQSTHTGEKP FGCEECGKAF STFSYLVQHQ RIHTSEKPYE CKECGKAFST
SSPLAKHQRI HTGEKPYECK ECGKSFTVYG QLTRHQSIHT GEKPFECKEC GKAFRLSSFL
HAHQRIHAEI KPYGCKECGK TFSRASYLVQ HGRLHTGEKP YECKECGKAF STGSYLVQHQ
RIHTGEKPYE CKECGKAFIS RHQLTVHQRV HTGEKPYECK ECGKAFRVHV HLTQHRKIHT
DVKPYECKEC GKTFSRASYL VQHSRIHTGK KPYECKECGK AFSSGSYLVQ HQRIHTGEKP
YECNKCGKAF TVYGQLIGHQ SVHTGEKPFE CKECGKAFRL NSFLTEHQRV HTGEKPFKCK
KCGKTFRYSS ALKVHLRKHM SVIP*

speed

0.31 s

All positions are in basepairs (bp) if not explicitly stated differently.
AA/aa: amino acid; CDS: coding sequence; mu: mutated; NMD: nonsense-mediated mRNA decay; nt: nucleotide; wt: wildtype; TGP: 1000 Genomes Project
back to results table

mutation t@sting

documentation

Prediction

polymorphism

Model: simple_aae, prob: 0.999998764803806 (classification due to TGP/ExAC, real probability is shown anyway) (explain)

Summary

amino acid sequence changed
homozygous in TGP or ExAC
protein features (might be) affected
splice site changes

hyperlink

analysed issue

analysis result

name of alteration

no title

alteration (phys. location)

chr19:35435006G>AN/A show variant in all transcripts IGV

HGNC symbol

ZNF30

Ensembl transcript ID

ENST00000303586

Genbank transcript ID

NM_001099437

UniProt peptide

P17039

alteration type

single base exchange

alteration region

CDS

DNA changes

c.1136G>A
cDNA.1514G>A
g.17200G>A

AA changes

R379K Score: 26 explain score(s)

position(s) of altered AA
if AA alteration in CDS

379

frameshift

known variant

Reference ID: rs1345658

database	homozygous (A/A)	heterozygous	allele carriers
1000G	1177	913	2090
ExAC	18346	-3925	14421

regulatory features

phyloP / phastCons

	PhyloP	PhastCons
(flanking)	3.262	0.897
	1.006	0.723
(flanking)	-0.446	0.062

explain score(s) and/or inspect your position(s) in in UCSC Genome Browser

splice sites

effect	gDNA position	score	wt detection sequence	exon-intron border
Donor increased	17203	wt: 0.34 / mu: 0.50	wt: GAGAACCTTCAGTCG mu: GAAAACCTTCAGTCG	GAAC\|cttc
Donor marginally increased	17197	wt: 0.5566 / mu: 0.5774 (marginal change - not scored)	wt: ATGCGGGAGAACCTT mu: ATGCGGGAAAACCTT	GCGG\|gaga
Donor gained	17195	0.92	mu: GAATGCGGGAAAACC	ATGC\|ggga
Donor gained	17196	0.90	mu: AATGCGGGAAAACCT	TGCG\|ggaa

distance from splice site

880

Kozak consensus sequence altered?

N/A

conservation
protein level for non-synonymous changes

species

match

gene

alignment

Human

379

mutated

all conserved

379

Ptroglodytes

all conserved

ENSPTRG00000010822

379

Mmulatta

no homologue

Fcatus

no homologue

Mmusculus

no homologue

Ggallus

no homologue

Trubripes

no homologue

Drerio

no homologue

Dmelanogaster

no homologue

Celegans

no homologue

Xtropicalis

no homologue

protein features

start (aa)	end (aa)	feature	details
372	394	ZN_FING	C2H2-type 10.	lost
400	422	ZN_FING	C2H2-type 11.	might get lost (downstream of altered splice site)
428	450	ZN_FING	C2H2-type 12.	might get lost (downstream of altered splice site)
456	478	ZN_FING	C2H2-type 13.	might get lost (downstream of altered splice site)
484	506	ZN_FING	C2H2-type 14.	might get lost (downstream of altered splice site)
492	492	CONFLICT	T -> A (in Ref. 4; AAI42997).	might get lost (downstream of altered splice site)
493	493	CONFLICT	F -> L (in Ref. 2; CAE45802).	might get lost (downstream of altered splice site)
512	534	ZN_FING	C2H2-type 15.	might get lost (downstream of altered splice site)
540	562	ZN_FING	C2H2-type 16.	might get lost (downstream of altered splice site)
568	590	ZN_FING	C2H2-type 17.	might get lost (downstream of altered splice site)
596	618	ZN_FING	C2H2-type 18.	might get lost (downstream of altered splice site)

length of protein

normal

AA sequence altered

yes

position of stopcodon in wt / mu CDS

1872 / 1872

position (AA) of stopcodon in wt / mu AA sequence

624 / 624

position of stopcodon in wt / mu cDNA

2250 / 2250

poly(A) signal

N/A

conservation
nucleotide level for all changes - no scoring up to now

N/A

position of start ATG in wt / mu cDNA

379 / 379

chromosome

strand

last intron/exon boundary

635

theoretical NMD boundary in CDS

206

length of CDS

1872

coding sequence (CDS) position

1136

cDNA position
(for ins/del: last normal base / first normal base)

1514

gDNA position
(for ins/del: last normal base / first normal base)

17200

chromosomal position
(for ins/del: last normal base / first normal base)

35435006

original gDNA sequence snippet

CGGATGCAAGGAATGCGGGAGAACCTTCAGTCGTGCCTCAT

altered gDNA sequence snippet

CGGATGCAAGGAATGCGGGAAAACCTTCAGTCGTGCCTCAT

original cDNA sequence snippet

CGGATGCAAGGAATGCGGGAGAACCTTCAGTCGTGCCTCAT

altered cDNA sequence snippet

CGGATGCAAGGAATGCGGGAAAACCTTCAGTCGTGCCTCAT

wildtype AA sequence

MAHKYVGLQY HGSVTFEDVA IAFSQQEWES LDSSQRGLYR DVMLENYRNL VSMGHSRSKP
HVIALLEQWK EPEVTVRKDG RRWCTDLQLE DDTIGCKEMP TSENCPSFAL HQKISRQKPR
ECQEYGKTLC QDSKPVQHER IHSSEKPNRC KECGKNFSNG HQLTIHQRLH VGEKPYKYEK
CGKAFISGSA FVKHGRIHTG EKPLKCKQCG KTISGSYQLT VHKSIHTGKK PYECGECGKA
FLVYGKLTRH QSTHTGEKPF GCEECGKAFS TFSYLVQHQR IHTSEKPYEC KECGKAFSTS
SPLAKHQRIH TGEKPYECKE CGKSFTVYGQ LTRHQSIHTG EKPFECKECG KAFRLSSFLH
AHQRIHAEIK PYGCKECGRT FSRASYLVQH GRLHTGEKPY ECKECGKAFS TGSYLVQHQR
IHTGEKPYEC KECGKAFISR HQLTVHQRVH TGEKPYECKE CGKAFRVHVH LTQHRKIHTD
VKPYECKECG KTFSRASYLV QHSRIHTGKK PYECKECGKA FSSGSYLVQH QRIHTGEKPY
ECNKCGKAFT VYGQLIGHQS VHTGEKPFEC KECGKAFRLN SFLTEHQRVH TGEKPFKCKK
CGKTFRYSSA LKVHLRKHMS VIP*

mutated AA sequence

MAHKYVGLQY HGSVTFEDVA IAFSQQEWES LDSSQRGLYR DVMLENYRNL VSMGHSRSKP
HVIALLEQWK EPEVTVRKDG RRWCTDLQLE DDTIGCKEMP TSENCPSFAL HQKISRQKPR
ECQEYGKTLC QDSKPVQHER IHSSEKPNRC KECGKNFSNG HQLTIHQRLH VGEKPYKYEK
CGKAFISGSA FVKHGRIHTG EKPLKCKQCG KTISGSYQLT VHKSIHTGKK PYECGECGKA
FLVYGKLTRH QSTHTGEKPF GCEECGKAFS TFSYLVQHQR IHTSEKPYEC KECGKAFSTS
SPLAKHQRIH TGEKPYECKE CGKSFTVYGQ LTRHQSIHTG EKPFECKECG KAFRLSSFLH
AHQRIHAEIK PYGCKECGKT FSRASYLVQH GRLHTGEKPY ECKECGKAFS TGSYLVQHQR
IHTGEKPYEC KECGKAFISR HQLTVHQRVH TGEKPYECKE CGKAFRVHVH LTQHRKIHTD
VKPYECKECG KTFSRASYLV QHSRIHTGKK PYECKECGKA FSSGSYLVQH QRIHTGEKPY
ECNKCGKAFT VYGQLIGHQS VHTGEKPFEC KECGKAFRLN SFLTEHQRVH TGEKPFKCKK
CGKTFRYSSA LKVHLRKHMS VIP*

speed

0.16 s

mutation t@sting

documentation

Prediction

polymorphism

Model: simple_aae, prob: 0.999998764803806 (classification due to TGP/ExAC, real probability is shown anyway) (explain)

Summary

amino acid sequence changed
homozygous in TGP or ExAC
protein features (might be) affected
splice site changes

hyperlink

analysed issue

analysis result

name of alteration

no title

alteration (phys. location)

chr19:35435006G>AN/A show variant in all transcripts IGV

HGNC symbol

ZNF30

Ensembl transcript ID

ENST00000426813

Genbank transcript ID

N/A

UniProt peptide

P17039

alteration type

single base exchange

alteration region

CDS

DNA changes

c.893G>A
cDNA.1449G>A
g.17200G>A

AA changes

R298K Score: 26 explain score(s)

position(s) of altered AA
if AA alteration in CDS

298

frameshift

known variant

Reference ID: rs1345658

database	homozygous (A/A)	heterozygous	allele carriers
1000G	1177	913	2090
ExAC	18346	-3925	14421

regulatory features

phyloP / phastCons

	PhyloP	PhastCons
(flanking)	3.262	0.897
	1.006	0.723
(flanking)	-0.446	0.062

explain score(s) and/or inspect your position(s) in in UCSC Genome Browser

splice sites

effect	gDNA position	score	wt detection sequence	exon-intron border
Donor increased	17203	wt: 0.34 / mu: 0.50	wt: GAGAACCTTCAGTCG mu: GAAAACCTTCAGTCG	GAAC\|cttc
Donor marginally increased	17197	wt: 0.5566 / mu: 0.5774 (marginal change - not scored)	wt: ATGCGGGAGAACCTT mu: ATGCGGGAAAACCTT	GCGG\|gaga
Donor gained	17195	0.92	mu: GAATGCGGGAAAACC	ATGC\|ggga
Donor gained	17196	0.90	mu: AATGCGGGAAAACCT	TGCG\|ggaa

distance from splice site

880

Kozak consensus sequence altered?

N/A

conservation
protein level for non-synonymous changes

species

match

gene

alignment

Human

298

mutated

all conserved

298

Ptroglodytes

all conserved

ENSPTRG00000010822

379

Mmulatta

no homologue

Fcatus

no homologue

Mmusculus

no homologue

Ggallus

no homologue

Trubripes

no homologue

Drerio

no homologue

Dmelanogaster

no homologue

Celegans

no homologue

Xtropicalis

no homologue

protein features

start (aa)	end (aa)	feature	details
288	310	ZN_FING	C2H2-type 7.	lost
304	304	CONFLICT	A -> V (in Ref. 2; CAE45802).	might get lost (downstream of altered splice site)
316	338	ZN_FING	C2H2-type 8.	might get lost (downstream of altered splice site)
344	366	ZN_FING	C2H2-type 9.	might get lost (downstream of altered splice site)
372	394	ZN_FING	C2H2-type 10.	might get lost (downstream of altered splice site)
400	422	ZN_FING	C2H2-type 11.	might get lost (downstream of altered splice site)
428	450	ZN_FING	C2H2-type 12.	might get lost (downstream of altered splice site)
456	478	ZN_FING	C2H2-type 13.	might get lost (downstream of altered splice site)
484	506	ZN_FING	C2H2-type 14.	might get lost (downstream of altered splice site)
492	492	CONFLICT	T -> A (in Ref. 4; AAI42997).	might get lost (downstream of altered splice site)
493	493	CONFLICT	F -> L (in Ref. 2; CAE45802).	might get lost (downstream of altered splice site)
512	534	ZN_FING	C2H2-type 15.	might get lost (downstream of altered splice site)
540	562	ZN_FING	C2H2-type 16.	might get lost (downstream of altered splice site)
568	590	ZN_FING	C2H2-type 17.	might get lost (downstream of altered splice site)
596	618	ZN_FING	C2H2-type 18.	might get lost (downstream of altered splice site)

length of protein

normal

AA sequence altered

yes

position of stopcodon in wt / mu CDS

1629 / 1629

position (AA) of stopcodon in wt / mu AA sequence

543 / 543

position of stopcodon in wt / mu cDNA

2185 / 2185

poly(A) signal

N/A

conservation
nucleotide level for all changes - no scoring up to now

N/A

position of start ATG in wt / mu cDNA

557 / 557

chromosome

strand

last intron/exon boundary

570

theoretical NMD boundary in CDS

cannot be calculated, too little distance between start ATG and last intron/exon boundary

length of CDS

1629

coding sequence (CDS) position

893

cDNA position
(for ins/del: last normal base / first normal base)

1449

gDNA position
(for ins/del: last normal base / first normal base)

17200

chromosomal position
(for ins/del: last normal base / first normal base)

35435006

original gDNA sequence snippet

CGGATGCAAGGAATGCGGGAGAACCTTCAGTCGTGCCTCAT

altered gDNA sequence snippet

CGGATGCAAGGAATGCGGGAAAACCTTCAGTCGTGCCTCAT

original cDNA sequence snippet

CGGATGCAAGGAATGCGGGAGAACCTTCAGTCGTGCCTCAT

altered cDNA sequence snippet

CGGATGCAAGGAATGCGGGAAAACCTTCAGTCGTGCCTCAT

wildtype AA sequence

MEPKDLQLED DTIGCKEMPT SENCPSFALH QKISRQKPRE CQEYGKTLCQ DSKPVQHERI
HSSEKPNRCK ECGKNFSNGH QLTIHQRLHV GEKPYKYEKC GKAFISGSAF VKHGRIHTGE
KPLKCKQCGK TISGSYQLTV HKSIHTGKKP YECGECGKAF LVYGKLTRHQ STHTGEKPFG
CEECGKAFST FSYLVQHQRI HTSEKPYECK ECGKAFSTSS PLAKHQRIHT GEKPYECKEC
GKSFTVYGQL TRHQSIHTGE KPFECKECGK AFRLSSFLHA HQRIHAEIKP YGCKECGRTF
SRASYLVQHG RLHTGEKPYE CKECGKAFST GSYLVQHQRI HTGEKPYECK ECGKAFISRH
QLTVHQRVHT GEKPYECKEC GKAFRVHVHL TQHRKIHTDV KPYECKECGK TFSRASYLVQ
HSRIHTGKKP YECKECGKAF SSGSYLVQHQ RIHTGEKPYE CNKCGKAFTV YGQLIGHQSV
HTGEKPFECK ECGKAFRLNS FLTEHQRVHT GEKPFKCKKC GKTFRYSSAL KVHLRKHMSV
IP*

mutated AA sequence

MEPKDLQLED DTIGCKEMPT SENCPSFALH QKISRQKPRE CQEYGKTLCQ DSKPVQHERI
HSSEKPNRCK ECGKNFSNGH QLTIHQRLHV GEKPYKYEKC GKAFISGSAF VKHGRIHTGE
KPLKCKQCGK TISGSYQLTV HKSIHTGKKP YECGECGKAF LVYGKLTRHQ STHTGEKPFG
CEECGKAFST FSYLVQHQRI HTSEKPYECK ECGKAFSTSS PLAKHQRIHT GEKPYECKEC
GKSFTVYGQL TRHQSIHTGE KPFECKECGK AFRLSSFLHA HQRIHAEIKP YGCKECGKTF
SRASYLVQHG RLHTGEKPYE CKECGKAFST GSYLVQHQRI HTGEKPYECK ECGKAFISRH
QLTVHQRVHT GEKPYECKEC GKAFRVHVHL TQHRKIHTDV KPYECKECGK TFSRASYLVQ
HSRIHTGKKP YECKECGKAF SSGSYLVQHQ RIHTGEKPYE CNKCGKAFTV YGQLIGHQSV
HTGEKPFECK ECGKAFRLNS FLTEHQRVHT GEKPFKCKKC GKTFRYSSAL KVHLRKHMSV
IP*

speed

0.31 s

MutationTaster - study a chromosomal position

Results

Taster files

mutation t@sting

Prediction

polymorphism

mutation t@sting

Prediction

polymorphism

mutation t@sting

Prediction

polymorphism

Problems