MutationTaster - study a chromosomal position

NEVER press reload or F5 - unless you want to start from the very beginning.
input seems to be ok - now mapping the variant to the different transcripts...
found 3 transcript(s)...
Querying Taster for transcript #1: ENST00000561234
Querying Taster for transcript #2: ENST00000416165
Querying Taster for transcript #3: ENST00000560846
MT speed 0 s - this script 3.493985 s

Results

genesymbol	prediction	probability	model	prediction problem	splicing	ClinVar	amino acid changes	variant type	dbSNP ID	protein length	file
BAHD1	polymorphism_automatic	0.035975797499019	simple_aae		affected		Q298K	single base exchange	rs3803357		show file
BAHD1	polymorphism_automatic	0.035975797499019	simple_aae		affected		Q298K	single base exchange	rs3803357		show file
BAHD1	polymorphism_automatic	0.035975797499019	simple_aae		affected		Q298K	single base exchange	rs3803357		show file

Taster files

mutation t@sting

documentation

Prediction

polymorphism

Model: simple_aae, prob: 0.964024202500981 (classification due to TGP/ExAC, real probability is shown anyway) (explain)

Summary

amino acid sequence changed
homozygous in TGP or ExAC
protein features (might be) affected
splice site changes

hyperlink

analysed issue

analysis result

name of alteration

no title

alteration (phys. location)

chr15:40751555C>AN/A show variant in all transcripts IGV

HGNC symbol

BAHD1

Ensembl transcript ID

ENST00000416165

Genbank transcript ID

NM_014952

UniProt peptide

Q8TBE0

alteration type

single base exchange

alteration region

CDS

DNA changes

c.892C>A
cDNA.963C>A
g.19636C>A

AA changes

Q298K Score: 53 explain score(s)

position(s) of altered AA
if AA alteration in CDS

298

frameshift

known variant

Reference ID: rs3803357

database	homozygous (A/A)	heterozygous	allele carriers
1000G	1213	958	2171
ExAC	21580	-10393	11187

regulatory features

H2BK5me1, Histone, Histone 2B Lysine 5 mono-methylation
H3K36me3, Histone, Histone 3 Lysine 36 Tri-Methylation
H4K20me1, Histone, Histone 4 Lysine 20 mono-methylation

phyloP / phastCons

	PhyloP	PhastCons
(flanking)	-0.011	0.164
	1.226	0.848
(flanking)	2.79	1

explain score(s) and/or inspect your position(s) in in UCSC Genome Browser

splice sites

effect	gDNA position	score	wt detection sequence	exon-intron border
Donor increased	19640	wt: 0.55 / mu: 0.68	wt: TCAGCCCCTGAGCAA mu: TAAGCCCCTGAGCAA	AGCC\|cctg
Donor gained	19632	0.98	mu: CCATCTCATAAGCCC	ATCT\|cata

distance from splice site

541

Kozak consensus sequence altered?

N/A

conservation
protein level for non-synonymous changes

species

match

gene

alignment

Human

298

mutated

all conserved

298

Ptroglodytes

all identical

ENSPTRG00000023028

298

Mmulatta

all identical

ENSMMUG00000017165

298

Fcatus

all identical

ENSFCAG00000013070

298

Mmusculus

all identical

ENSMUSG00000040007

296

Ggallus

all conserved

ENSGALG00000004658

Trubripes

not conserved

ENSTRUG00000010329

387

Drerio

no alignment

ENSDARG00000090712

n/a

Dmelanogaster

no alignment

FBgn0052529

n/a

Celegans

no homologue

Xtropicalis

all conserved

ENSXETG00000016075

250

protein features

start (aa)	end (aa)	feature	details
239	361	COMPBIAS	Pro-rich.	lost
556	587	COMPBIAS	Arg-rich.	might get lost (downstream of altered splice site)
624	779	DOMAIN	BAH.	might get lost (downstream of altered splice site)

length of protein

normal

AA sequence altered

yes

position of stopcodon in wt / mu CDS

2343 / 2343

position (AA) of stopcodon in wt / mu AA sequence

781 / 781

position of stopcodon in wt / mu cDNA

2414 / 2414

poly(A) signal

N/A

conservation
nucleotide level for all changes - no scoring up to now

N/A

position of start ATG in wt / mu cDNA

72 / 72

chromosome

strand

last intron/exon boundary

2225

theoretical NMD boundary in CDS

2103

length of CDS

2343

coding sequence (CDS) position

892

cDNA position
(for ins/del: last normal base / first normal base)

963

gDNA position
(for ins/del: last normal base / first normal base)

19636

chromosomal position
(for ins/del: last normal base / first normal base)

40751555

original gDNA sequence snippet

CATCCGTCCAGCCATCTCATCAGCCCCTGAGCAAGGCTCTG

altered gDNA sequence snippet

CATCCGTCCAGCCATCTCATAAGCCCCTGAGCAAGGCTCTG

original cDNA sequence snippet

CATCCGTCCAGCCATCTCATCAGCCCCTGAGCAAGGCTCTG

altered cDNA sequence snippet

CATCCGTCCAGCCATCTCATAAGCCCCTGAGCAAGGCTCTG

wildtype AA sequence

MTHTRRKSLP MLSSGLTGRR EPLQMEDSNM EQGVEGVEPG MPESPGHLTG RRKNYPLRKR
PLVPEKPKAC KVLLTRLENV AGPRSADEAD ELPPDLPKPP SPAPSSEDPG LAQPRKRRLA
SLNAEALNNL LLEREDTSSL AGTRRSRAGD PHRSRDRDRA TGGWSSSKKR PRLGDLGGGS
RDLSPEPAPD EGPRRDGDPA PKRLASLNAA AFLKLSQERE LPLRLPRAHA EVDGRSTEPP
APKAPRPKWP KVNGKNYPKA WQGASSGEAA GPPGWQGCPD EPWPSATPCG PSVQPSHQPL
SKALESPLGL RPHLPLLMGG QAALKPEPGR PGEESPAPKQ ELHQPSFPTP QLSPLPMPGN
PADYNGLCVG PELTALGSFY LYCGQEGLQC GGYSPCPMLP EGKLSPVAAP HEEGLLLAPS
SVPSGTPFQH PPWGSSRYCS SEDTGVNGYS ICGVLPLSVT HAGTTCGGCP YKMPFAAEGC
RSLGQLEFPL PEAGHPASPA HPLLGCPVPS VPPAAEPVPH LQTPTSEPQT VARACPQSAK
PPSGSKSGLR TGSSCRHTAR SKAARRPSHP KQPRVQRPRP RRRRRRRTNG WVPVGAACEK
AVYVLDEPEP AIRKSYQAVE RHGETIRVRD TVLLKSGPRK TSTPYVAKIS ALWENPESGE
LMMSLLWYYR PEHLQGGRSP SMHEPLQNEV FASRHQDQNS VACIEEKCYV LTFAEYCRFC
AMAKRRGEGL PSRKTALVPP SADYSTPPHR TVPEDTDPEL VFLCRHVYDF RHGRILKNPQ
*

mutated AA sequence

MTHTRRKSLP MLSSGLTGRR EPLQMEDSNM EQGVEGVEPG MPESPGHLTG RRKNYPLRKR
PLVPEKPKAC KVLLTRLENV AGPRSADEAD ELPPDLPKPP SPAPSSEDPG LAQPRKRRLA
SLNAEALNNL LLEREDTSSL AGTRRSRAGD PHRSRDRDRA TGGWSSSKKR PRLGDLGGGS
RDLSPEPAPD EGPRRDGDPA PKRLASLNAA AFLKLSQERE LPLRLPRAHA EVDGRSTEPP
APKAPRPKWP KVNGKNYPKA WQGASSGEAA GPPGWQGCPD EPWPSATPCG PSVQPSHKPL
SKALESPLGL RPHLPLLMGG QAALKPEPGR PGEESPAPKQ ELHQPSFPTP QLSPLPMPGN
PADYNGLCVG PELTALGSFY LYCGQEGLQC GGYSPCPMLP EGKLSPVAAP HEEGLLLAPS
SVPSGTPFQH PPWGSSRYCS SEDTGVNGYS ICGVLPLSVT HAGTTCGGCP YKMPFAAEGC
RSLGQLEFPL PEAGHPASPA HPLLGCPVPS VPPAAEPVPH LQTPTSEPQT VARACPQSAK
PPSGSKSGLR TGSSCRHTAR SKAARRPSHP KQPRVQRPRP RRRRRRRTNG WVPVGAACEK
AVYVLDEPEP AIRKSYQAVE RHGETIRVRD TVLLKSGPRK TSTPYVAKIS ALWENPESGE
LMMSLLWYYR PEHLQGGRSP SMHEPLQNEV FASRHQDQNS VACIEEKCYV LTFAEYCRFC
AMAKRRGEGL PSRKTALVPP SADYSTPPHR TVPEDTDPEL VFLCRHVYDF RHGRILKNPQ
*

speed

0.87 s

All positions are in basepairs (bp) if not explicitly stated differently.
AA/aa: amino acid; CDS: coding sequence; mu: mutated; NMD: nonsense-mediated mRNA decay; nt: nucleotide; wt: wildtype; TGP: 1000 Genomes Project
back to results table

mutation t@sting

documentation

Prediction

polymorphism

Model: simple_aae, prob: 0.964024202500981 (classification due to TGP/ExAC, real probability is shown anyway) (explain)

Summary

amino acid sequence changed
homozygous in TGP or ExAC
protein features (might be) affected
splice site changes

hyperlink

analysed issue

analysis result

name of alteration

no title

alteration (phys. location)

chr15:40751555C>AN/A show variant in all transcripts IGV

HGNC symbol

BAHD1

Ensembl transcript ID

ENST00000561234

Genbank transcript ID

N/A

UniProt peptide

Q8TBE0

alteration type

single base exchange

alteration region

CDS

DNA changes

c.892C>A
cDNA.1151C>A
g.19636C>A

AA changes

Q298K Score: 53 explain score(s)

position(s) of altered AA
if AA alteration in CDS

298

frameshift

known variant

Reference ID: rs3803357

database	homozygous (A/A)	heterozygous	allele carriers
1000G	1213	958	2171
ExAC	21580	-10393	11187

regulatory features

H2BK5me1, Histone, Histone 2B Lysine 5 mono-methylation
H3K36me3, Histone, Histone 3 Lysine 36 Tri-Methylation
H4K20me1, Histone, Histone 4 Lysine 20 mono-methylation

phyloP / phastCons

	PhyloP	PhastCons
(flanking)	-0.011	0.164
	1.226	0.848
(flanking)	2.79	1

explain score(s) and/or inspect your position(s) in in UCSC Genome Browser

splice sites

effect	gDNA position	score	wt detection sequence	exon-intron border
Donor increased	19640	wt: 0.55 / mu: 0.68	wt: TCAGCCCCTGAGCAA mu: TAAGCCCCTGAGCAA	AGCC\|cctg
Donor gained	19632	0.98	mu: CCATCTCATAAGCCC	ATCT\|cata

distance from splice site

541

Kozak consensus sequence altered?

N/A

conservation
protein level for non-synonymous changes

species

match

gene

alignment

Human

298

mutated

all conserved

298

Ptroglodytes

all identical

ENSPTRG00000023028

298

Mmulatta

all identical

ENSMMUG00000017165

298

Fcatus

all identical

ENSFCAG00000013070

298

Mmusculus

all identical

ENSMUSG00000040007

296

Ggallus

all conserved

ENSGALG00000004658

Trubripes

not conserved

ENSTRUG00000010329

387

Drerio

no alignment

ENSDARG00000090712

n/a

Dmelanogaster

no alignment

FBgn0052529

n/a

Celegans

no homologue

Xtropicalis

all conserved

ENSXETG00000016075

250

protein features

start (aa)	end (aa)	feature	details
239	361	COMPBIAS	Pro-rich.	lost
556	587	COMPBIAS	Arg-rich.	might get lost (downstream of altered splice site)
624	779	DOMAIN	BAH.	might get lost (downstream of altered splice site)

length of protein

normal

AA sequence altered

yes

position of stopcodon in wt / mu CDS

2340 / 2340

position (AA) of stopcodon in wt / mu AA sequence

780 / 780

position of stopcodon in wt / mu cDNA

2599 / 2599

poly(A) signal

N/A

conservation
nucleotide level for all changes - no scoring up to now

N/A

position of start ATG in wt / mu cDNA

260 / 260

chromosome

strand

last intron/exon boundary

2410

theoretical NMD boundary in CDS

2100

length of CDS

2340

coding sequence (CDS) position

892

cDNA position
(for ins/del: last normal base / first normal base)

1151

gDNA position
(for ins/del: last normal base / first normal base)

19636

chromosomal position
(for ins/del: last normal base / first normal base)

40751555

original gDNA sequence snippet

CATCCGTCCAGCCATCTCATCAGCCCCTGAGCAAGGCTCTG

altered gDNA sequence snippet

CATCCGTCCAGCCATCTCATAAGCCCCTGAGCAAGGCTCTG

original cDNA sequence snippet

CATCCGTCCAGCCATCTCATCAGCCCCTGAGCAAGGCTCTG

altered cDNA sequence snippet

CATCCGTCCAGCCATCTCATAAGCCCCTGAGCAAGGCTCTG

wildtype AA sequence

MTHTRRKSLP MLSSGLTGRR EPLQMEDSNM EQGVEGVEPG MPESPGHLTG RRKNYPLRKR
PLVPEKPKAC KVLLTRLENV AGPRSADEAD ELPPDLPKPP SPAPSSEDPG LAQPRKRRLA
SLNAEALNNL LLEREDTSSL AGTRRSRAGD PHRSRDRDRA TGGWSSSKKR PRLGDLGGGS
RDLSPEPAPD EGPRRDGDPA PKRLASLNAA AFLKLSQERE LPLRLPRAHA EVDGRSTEPP
APKAPRPKWP KVNGKNYPKA WQGASSGEAA GPPGWQGCPD EPWPSATPCG PSVQPSHQPL
SKALESPLGL RPHLPLLMGG QAALKPEPGR PGEESPAPKQ ELHQPSFPTP QLSPLPMPGN
PADYNGLCVG PELTALGSFY LYCGQEGLQC GGYSPCPMLP EGKLSPVAAP HEEGLLLAPS
SVPSGTPFQH PPWGSSRYCS SEDTGVNGYS ICGVLPLSVT HAGTTCGGCP YKMPFAAGCR
SLGQLEFPLP EAGHPASPAH PLLGCPVPSV PPAAEPVPHL QTPTSEPQTV ARACPQSAKP
PSGSKSGLRT GSSCRHTARS KAARRPSHPK QPRVQRPRPR RRRRRRTNGW VPVGAACEKA
VYVLDEPEPA IRKSYQAVER HGETIRVRDT VLLKSGPRKT STPYVAKISA LWENPESGEL
MMSLLWYYRP EHLQGGRSPS MHEPLQNEVF ASRHQDQNSV ACIEEKCYVL TFAEYCRFCA
MAKRRGEGLP SRKTALVPPS ADYSTPPHRT VPEDTDPELV FLCRHVYDFR HGRILKNPQ*

mutated AA sequence

MTHTRRKSLP MLSSGLTGRR EPLQMEDSNM EQGVEGVEPG MPESPGHLTG RRKNYPLRKR
PLVPEKPKAC KVLLTRLENV AGPRSADEAD ELPPDLPKPP SPAPSSEDPG LAQPRKRRLA
SLNAEALNNL LLEREDTSSL AGTRRSRAGD PHRSRDRDRA TGGWSSSKKR PRLGDLGGGS
RDLSPEPAPD EGPRRDGDPA PKRLASLNAA AFLKLSQERE LPLRLPRAHA EVDGRSTEPP
APKAPRPKWP KVNGKNYPKA WQGASSGEAA GPPGWQGCPD EPWPSATPCG PSVQPSHKPL
SKALESPLGL RPHLPLLMGG QAALKPEPGR PGEESPAPKQ ELHQPSFPTP QLSPLPMPGN
PADYNGLCVG PELTALGSFY LYCGQEGLQC GGYSPCPMLP EGKLSPVAAP HEEGLLLAPS
SVPSGTPFQH PPWGSSRYCS SEDTGVNGYS ICGVLPLSVT HAGTTCGGCP YKMPFAAGCR
SLGQLEFPLP EAGHPASPAH PLLGCPVPSV PPAAEPVPHL QTPTSEPQTV ARACPQSAKP
PSGSKSGLRT GSSCRHTARS KAARRPSHPK QPRVQRPRPR RRRRRRTNGW VPVGAACEKA
VYVLDEPEPA IRKSYQAVER HGETIRVRDT VLLKSGPRKT STPYVAKISA LWENPESGEL
MMSLLWYYRP EHLQGGRSPS MHEPLQNEVF ASRHQDQNSV ACIEEKCYVL TFAEYCRFCA
MAKRRGEGLP SRKTALVPPS ADYSTPPHRT VPEDTDPELV FLCRHVYDFR HGRILKNPQ*

speed

0.65 s

mutation t@sting

documentation

Prediction

polymorphism

Model: simple_aae, prob: 0.964024202500981 (classification due to TGP/ExAC, real probability is shown anyway) (explain)

Summary

amino acid sequence changed
homozygous in TGP or ExAC
protein features (might be) affected
splice site changes

hyperlink

analysed issue

analysis result

name of alteration

no title

alteration (phys. location)

chr15:40751555C>AN/A show variant in all transcripts IGV

HGNC symbol

BAHD1

Ensembl transcript ID

ENST00000560846

Genbank transcript ID

N/A

UniProt peptide

Q8TBE0

alteration type

single base exchange

alteration region

CDS

DNA changes

c.892C>A
cDNA.892C>A
g.19636C>A

AA changes

Q298K Score: 53 explain score(s)

position(s) of altered AA
if AA alteration in CDS

298

frameshift

known variant

Reference ID: rs3803357

database	homozygous (A/A)	heterozygous	allele carriers
1000G	1213	958	2171
ExAC	21580	-10393	11187

regulatory features

H2BK5me1, Histone, Histone 2B Lysine 5 mono-methylation
H3K36me3, Histone, Histone 3 Lysine 36 Tri-Methylation
H4K20me1, Histone, Histone 4 Lysine 20 mono-methylation

phyloP / phastCons

	PhyloP	PhastCons
(flanking)	-0.011	0.164
	1.226	0.848
(flanking)	2.79	1

explain score(s) and/or inspect your position(s) in in UCSC Genome Browser

splice sites

effect	gDNA position	score	wt detection sequence	exon-intron border
Donor increased	19640	wt: 0.55 / mu: 0.68	wt: TCAGCCCCTGAGCAA mu: TAAGCCCCTGAGCAA	AGCC\|cctg
Donor gained	19632	0.98	mu: CCATCTCATAAGCCC	ATCT\|cata

distance from splice site

541

Kozak consensus sequence altered?

N/A

conservation
protein level for non-synonymous changes

species

match

gene

alignment

Human

298

mutated

all conserved

298

Ptroglodytes

all identical

ENSPTRG00000023028

298

Mmulatta

all identical

ENSMMUG00000017165

298

Fcatus

all identical

ENSFCAG00000013070

298

Mmusculus

all identical

ENSMUSG00000040007

296

Ggallus

all conserved

ENSGALG00000004658

Trubripes

not conserved

ENSTRUG00000010329

387

Drerio

no alignment

ENSDARG00000090712

n/a

Dmelanogaster

no alignment

FBgn0052529

n/a

Celegans

no homologue

Xtropicalis

all conserved

ENSXETG00000016075

250

protein features

start (aa)	end (aa)	feature	details
239	361	COMPBIAS	Pro-rich.	lost
556	587	COMPBIAS	Arg-rich.	might get lost (downstream of altered splice site)
624	779	DOMAIN	BAH.	might get lost (downstream of altered splice site)

length of protein

normal

AA sequence altered

yes

position of stopcodon in wt / mu CDS

2334 / 2334

position (AA) of stopcodon in wt / mu AA sequence

778 / 778

position of stopcodon in wt / mu cDNA

2334 / 2334

poly(A) signal

N/A

conservation
nucleotide level for all changes - no scoring up to now

N/A

position of start ATG in wt / mu cDNA

1 / 1

chromosome

strand

last intron/exon boundary

2145

theoretical NMD boundary in CDS

2094

length of CDS

2334

coding sequence (CDS) position

892

cDNA position
(for ins/del: last normal base / first normal base)

892

gDNA position
(for ins/del: last normal base / first normal base)

19636

chromosomal position
(for ins/del: last normal base / first normal base)

40751555

original gDNA sequence snippet

CATCCGTCCAGCCATCTCATCAGCCCCTGAGCAAGGCTCTG

altered gDNA sequence snippet

CATCCGTCCAGCCATCTCATAAGCCCCTGAGCAAGGCTCTG

original cDNA sequence snippet

CATCCGTCCAGCCATCTCATCAGCCCCTGAGCAAGGCTCTG

altered cDNA sequence snippet

CATCCGTCCAGCCATCTCATAAGCCCCTGAGCAAGGCTCTG

wildtype AA sequence

MTHTRRKSLP MLSSGLTGRR EPLQMEDSNM EQGVEGVEPG MPESPGHLTG RRKNYPLRKR
PLVPEKPKAC KVLLTRLENV AGPRSADEAD ELPPDLPKPP SPAPSSEDPG LAQPRKRRLA
SLNAEALNNL LLEREDTSSL AGTRRSRAGD PHRSRDRDRA TGGWSSSKKR PRLGDLGGGS
RDLSPEPAPD EGPRRDGDPA PKRLASLNAA AFLKLSQERE LPLRLPRAHA EVDGRSTEPP
APKAPRPKWP KVNGKNYPKA WQGASSGEAA GPPGWQGCPD EPWPSATPCG PSVQPSHQPL
SKALESPLGL RPHLPLLMGG QAALKPEPGR PGEESPAPKQ ELHQPSFPTP QLSPLPMPGN
PADYNGLCVG PELTALGSFY LYCGQEGLQC GGYSPCPMLP EGKLSPVAAP HEEGLLLAPS
SVPSGTPFQH PPWGSSRYCS SEDTGVNGYS ICGVLPLSVT HAGTTCGGCP YKMPFAAEGC
RSLGQLEFPL PEAGHPASPA HPLLGCPVPS VPPAAEPVPH LQTPTSEPQT VARACPQSAK
PPSGSKSGLR TGSSCRHTAR SKAARRPSHP KQPRVQRPRP RRRRRRRTNG WVPVGAACEK
AVYVLDEPEP AIRKSYQAVE RHGETIRVRD TVLLKSGPRK TSTPYVAKIS ALWENPESGE
LMMSLLWYYR PEHLQGGRSP SMHENEVFAS RHQDQNSVAC IEEKCYVLTF AEYCRFCAMA
KRRGEGLPSR KTALVPPSAD YSTPPHRTVP EDTDPELVFL CRHVYDFRHG RILKNPQ*

mutated AA sequence

MTHTRRKSLP MLSSGLTGRR EPLQMEDSNM EQGVEGVEPG MPESPGHLTG RRKNYPLRKR
PLVPEKPKAC KVLLTRLENV AGPRSADEAD ELPPDLPKPP SPAPSSEDPG LAQPRKRRLA
SLNAEALNNL LLEREDTSSL AGTRRSRAGD PHRSRDRDRA TGGWSSSKKR PRLGDLGGGS
RDLSPEPAPD EGPRRDGDPA PKRLASLNAA AFLKLSQERE LPLRLPRAHA EVDGRSTEPP
APKAPRPKWP KVNGKNYPKA WQGASSGEAA GPPGWQGCPD EPWPSATPCG PSVQPSHKPL
SKALESPLGL RPHLPLLMGG QAALKPEPGR PGEESPAPKQ ELHQPSFPTP QLSPLPMPGN
PADYNGLCVG PELTALGSFY LYCGQEGLQC GGYSPCPMLP EGKLSPVAAP HEEGLLLAPS
SVPSGTPFQH PPWGSSRYCS SEDTGVNGYS ICGVLPLSVT HAGTTCGGCP YKMPFAAEGC
RSLGQLEFPL PEAGHPASPA HPLLGCPVPS VPPAAEPVPH LQTPTSEPQT VARACPQSAK
PPSGSKSGLR TGSSCRHTAR SKAARRPSHP KQPRVQRPRP RRRRRRRTNG WVPVGAACEK
AVYVLDEPEP AIRKSYQAVE RHGETIRVRD TVLLKSGPRK TSTPYVAKIS ALWENPESGE
LMMSLLWYYR PEHLQGGRSP SMHENEVFAS RHQDQNSVAC IEEKCYVLTF AEYCRFCAMA
KRRGEGLPSR KTALVPPSAD YSTPPHRTVP EDTDPELVFL CRHVYDFRHG RILKNPQ*

speed

0.60 s

MutationTaster - study a chromosomal position

Results

Taster files

mutation t@sting

Prediction

polymorphism

mutation t@sting

Prediction

polymorphism

mutation t@sting

Prediction

polymorphism

Problems